Schizachyrium little blue stem grass12/28/2023 Ornamental grasses have become exceedingly popular in the United States as a result of gardeners’ acceptance of a more natural landscape appearance and the low maintenance requirements of ornamental grasses ( Meyer, 2012). Keywords: Schizachyrium scoparium ornamental grass SSR marker population structure ploidy These microsatellite markers are the first sequence-specific markers designed for little bluestem and can serve as a resource for future genetic studies. Furthermore, no variation in ploidy level was seen among little bluestem samples. The lowest genetic similarity between little bluestem samples was 86%, which was surprising as a high degree of morphological variation is seen in this species. Using the software program Structure, cluster analysis of the SSR data for S. The most frequently identified fragments were the long terminal repeat retrotransposons Gypsy (177 fragments) and Copia (98 fragments) and the DNA transposon EnSpm (60 fragments). The use of a repetitive sequence identifier found that of 117 examined sequences, only nine sequences did not have similarity to DNA transposons, retrotransposons, viruses, or satellite sequences. Twenty-two primer pairs generated more than four fragments per accession. Only 35 primer pairs, generating 102 scored fragments, were polymorphic among S. A total of 2812 primer sets was designed from high-throughput sequencing, 100 primer pairs were selected, and 82 of these primers successfully amplified DNA from the Schizachyrium accessions. Ten microsatellite markers, with successful amplification, were developed by using sequences available in Genbank and additional simple sequence repeat (SSR) markers were generated by using ion torrent sequencing of a genomic library created from the cultivar The Blues. Department of Agriculture National Plant Germplasm System collection. ![]() With the widespread planting of only a few cultivars, we aimed to assess the ploidy level and genetic diversity among some popular cultivars and accessions in the U.S. Originally used as a forage grass, little bluestem is now listed as a major U.S. Little bluestem ( Schizachyrium scoparium) is a perennial bunchgrass that is native to North American prairies and woodlands from southern Canada to northern Mexico.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |